Application

Research Sub CategoryTranscription Factors

Research CategoryStem Cell Research

Western Blotting Analysis: 1 µg/mL of this antibody from a representative lot detected both endogenous Grainyhead-Like 3/GRHL3 and a myc-tagged GRHL3 in 50 µg of lysate from transfected HEK293 cells.
Immunoprecipitation Analysis: 5.0 µg of this antibody from a representative lot immunorecipitated both the endogenous and an epitopically tagged human GRHL3 transiently expressed in HEK293 cells (Courtesy of Professor Judith Haendeler, IUF-Leibniz Research Institute, University of Duesseldorf, Germany).
Immunocytochemistry Analysis: 4.0 µg/mL of this antibody from a representative lot detected GRHL3 in paraformaldehyde-fixed, Triton X-100-permeablized HUVECs (Courtesy of Professor Judith Haendeler, IUF-Leibniz Research Institute, University of Duesseldorf, Germany).
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected both the endogenous and an epitopically tagged human GRHL3 transiently expressed in HEK293 cells (Courtesy of Professor Judith Haendeler, IUF-Leibniz Research Institute, University of Duesseldorf, Germany).

Anti-Grainyhead-Like 3 Antibody is an antibody against GRHL3 for use in Western Blotting, Immunoprecipitation, Immunocytochemistry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Grainyhead-like protein 3 homolog (UniProt Q8TE85; also known as Sister of mammalian grainyhead, Transcription factor CP2-like 4) is encoded by the GRHL3 (also known as SOM, TFCP2L4, VWS2) gene (Gene ID 57822) in human. Mammalian grainyhead-like (GRHL1, GRHL2, and GRHL3) genes code for transcription factors and are named after their drosophila homolog grainyhead (GRH). These factors show tissue- and developmental stage-dependent expression patterns. Grainyhead-like 3 (GRHL3) is reported to play a protective role against squamous cell carcinoma (SCC) of the skin. Tumor suppressor phosphatase and tensin homolog (PTEN) is a direct target of GRHL3, Grhl3-knockdown in mice causes skin tumor formation as a result of reduced Pten expression. Consistently, downregulated GRHL3 and PTEN levels are found in human SCC skin samples. In addition, GRHL3 is a positive regulator of E-cadherin expression during mesenchymal epithelial transition (MET) in non-tumorigenic mammary gland cells, as well as in breast and lung cancer cells. High GRHL3 expression is found in early stages of breast cancer and the expression is greatly reduced in advanced stages of breast cancer.

Immunogen

Epitope: Internal (C-terminal half).

KLH-conjugated linear peptide corresponding to an internal sequence from the C-terminal half of human Grainyhead-Like 3/GRHL3.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Grainyhead-Like 3/GRHL3 in 10 µg of Jurkat cell lysate.

Specificity

Expected to react with all five spliced isoforms of human grainyhead-like protein 3 reported by UniProt (Q8TE85).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~62 kDa observed. 70.35 kDa (isoform 1), 68.35 kDa (isoform 2), 62.38 kDa (isoform 3), 57.05 kDa (isoform 4), and 67.70 kDa (isoform 5) calculated. Uncharacterized band(s) may appear in some lysates.